Comparative evaluation of three assay systems for automated determination of hemoglobin A1c.

Evaluation of a nucleic acid-based cross-linking assay to screen for hereditary hemochromatosis in healthy blood donors.

14. 13. Chung BH, Segrest JP, Ray MJ, Brunzell JD, Hokanson JE, Krauss RM, et al. Single vertical spin density gradient ultracentrifugation. Methods Enzymol 1986;128:181–209. 14. Khuu HM, Robinson CA, Goolsby K, Hardy RW, Konrad RJ. Evaluation of a fully automated high-performance liquid chromatography assay for hemoglobin A1c. Arch Pathol Lab Med 1999;123:763–7. 15. Khuu HM, Robinson CA, Briss...

Correlation between Standard Clinical Chemistry and Point of Care Testing Analyzers on Hemoglobin A1C Determination

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c.

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contai...

Performance characteristics of three automated immunoassays for thyroid hormones

Background: Since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. We performed an analytical evaluation of the new electrochemiluminescent immunoassay (ECLIA) for serum TSH, FT4 and T3 in the Elecsys 2010 immunoassay system and compared the resu...

Hemoglobin A1c: Clinical applications and analytical considerations

Diabetes Mellitus (DM) is the most common disorder of carbohydrate metabolism. Hyperglycemia is the common feature of DM. Hyperglycemia causes cardiovascular, renal, ocular, and peripheral nerve disorders in patients with diabetes. Glycemic regulation plays an essential role in controlling these complications. Thus, evaluation of the glycemic control status is crucial in the management of DM. H...